Colocalization of Fluorescent Probes
ثبت نشده
چکیده
1. Introduction Fluorescence labeling techniques are quite useful to microscopists. Not only can fluorescent probes label sub-cellular structures with a high degree of specificity, they can also do so with a very high degree of sensitivity. This combination of specificity and sensitivity permits observations of cellular events that are not always visible using traditional methodologies, such as brightfield microscopy.
منابع مشابه
Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.
Colocalization of fluorescent probes is commonly used in cell biology to discern the proximity of two proteins in the cell. Considering that the resolution limit of optical microscopy is on the order of 250 nm, there has not been a need for high-resolution colocalization techniques. However, with the advent of higher resolution techniques for cell biology and single-molecule biophysics, colocal...
متن کاملUltrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.
An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the methodology, theoretical considerat...
متن کاملPerspectives in Cell Physiology Image acquisition for colocalization using optical microscopy
Scriven DR, Lynch RM, Moore ED. Image acquisition for colocalization using optical microscopy. Am J Physiol Cell Physiol 294: C1119–C1122, 2008. First published March 19, 2008; doi:10.1152/ajpcell.00133.2008.—Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common analytical tool in cell biology. I...
متن کاملUltrahigh-resolution multicolor colocalization of single fluorescent probes.
An optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described in this paper. It relies on the use of two unique families of fluorophores, namely energy-transfer fluorescent beads (TransFluoSpheres) and semiconductor nanocrystal quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A multicolor sample-scanning ...
متن کاملSingle molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time.
Here we present a technique called single-molecule high-resolution colocalization (SHREC) of fluorescent dyes that allows the measurement of interfluorophore distances in macromolecules and macromolecular complexes with better than 10-nm resolution. By using two chromatically differing fluorescent molecules as probes, we are able to circumvent the Rayleigh criterion and measure distances much s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2002